Edvotek Quantification of DNA Damage by qPCR [80256]

The integrity and stability of DNA is essential to life. However, every day this molecule is under assault from environmental stressors like UV radiation, mutagenic chemicals, and even normal metabolic processes. In this guided inquiry lab students will use the cutting edge technology of QPCR to investigate and quantify DNA damage due to physical (UV radiation) or chemical (DNAseI) disruptions. By designing and performing the experiments students will master advanced analytical and technical skills as well as deepen their understanding of key molecular biology and medical concepts. Expt. 381