Edvotek DNA Fingerprinting Using PCR Kit – Classroom Investigation

The Edvotek DNA Fingerprinting Using PCR Kit gives students a hands‑on opportunity to carry out Polymerase Chain Reaction (PCR) in the classroom while exploring real‑world applications of molecular biology. Designed for education, this engaging practical allows students to amplify DNA samples and analyse individual DNA profiles to solve a simulated crime.

Using provided plasmid DNA templates, students perform PCR to generate DNA fragments representing unique genetic fingerprints. These are then analysed using gel electrophoresis, enabling learners to compare samples, interpret results, and draw evidence‑based conclusions. Clear, step‑by‑step instructions support independent learning and teamwork throughout the investigation.

The full activity can be completed in approximately 2.5 hours and is suitable for up to 6 lab groups, with 2–4 students per group, making it ideal for structured practical lessons or enrichment activities.

Kit Contents

• Detailed student instructions
• PCR EdvoBeads™
• LyphoPrimer™ Mix
• DNA templates
• EdvoQuick™ DNA Ladder
• TE buffer
• UltraSpec‑Agarose™
• Electrophoresis buffer (50×)
• SYBR® Safe stain
• FlashBlue™ DNA stain
• Microcentrifuge tubes and PCR tubes

Additional equipment required: micropipettes (5–50 µl or fixed‑volume minipipettes), pipette tips, thermal cycler, electrophoresis tank, and power supply.

Key Features

• Hands‑on introduction to PCR and DNA fingerprinting
• Realistic forensic science crime‑scene scenario
• Student‑friendly, step‑by‑step experimental design
• Supports understanding of DNA amplification and analysis
• Suitable for 6 lab groups (2–4 students per group)
• Clear results using gel electrophoresis
• Designed specifically for educational laboratory use

Ideal for School Science & Biology Lessons

Ideal for GCSE, A‑Level, and advanced secondary biology, this DNA Fingerprinting Using PCR kit allows students to apply theoretical knowledge in a practical, investigative context while developing key laboratory skills.

• Teaching PCR and DNA amplification
• Demonstrating gel electrophoresis techniques
• Exploring forensic science and genetics
• Encouraging data analysis and critical thinking
• Supporting independent and collaborative learning

Engaging, informative, and curriculum‑relevant, the Edvotek DNA Fingerprinting Using PCR Kit brings modern molecular biology into the classroom and inspires students through real‑world scientific investigation.