Transformation of E. coli with Green Fluorescent Protein (GFP) Kit [1830]

The Transformation of E. coli with Green Fluorescent Protein Kit provides an exciting, hands‑on introduction to genetic engineering and recombinant DNA technology. In this practical investigation, students transform E. coli cells with a plasmid containing the Green Fluorescent Protein (GFP) gene, originally isolated from the jellyfish Aequorea victoria.

Successfully transformed bacterial colonies express the GFP protein, allowing students to directly observe gene expression. Colonies appear green under normal light and glow brightly when exposed to long‑wave UV light, creating a highly visual and memorable learning experience. This real‑world biotechnology experiment clearly demonstrates how genes can be transferred, expressed, and controlled in living organisms.

Designed for classroom use, the kit supports up to 10 laboratory groups and includes sterile, ready‑to‑use components for reliable results. It offers an excellent opportunity for students to develop confidence with microbiological techniques while reinforcing key molecular biology concepts.

Kit Contents

• Comprehensive teacher and student instructions
• BactoBeads™
• E. coli GFP host cells
• Supercoiled pFluoroGreen™ plasmid
• Ampicillin and IPTG
• Calcium chloride (CaCl₂)
• ReadyPour™ Luria Broth Agar (sterile)
• Luria Broth recovery medium (sterile)
• Small and large Petri plates
• Plastic micro‑tipped transfer pipettes
• Wrapped 10 ml sterile pipette
• Sterile toothpicks and inoculating loops
• Microcentrifuge tubes
• Materials for 10 lab groups

Additional Equipment Required

• Automatic micropipette (5–50 µl) and tips
• Two water baths (37 °C and 42 °C)
• Incubation oven (37 °C)
• Thermometer
• Pipette pumps or bulbs
• Ice and marking pens
• Bunsen burner
• Hot plate or microwave
• Heat‑resistant gloves
• Long‑wave UV light

Ideal for School Science Lessons

Ideal for GCSE and A‑Level Biology, this GFP transformation kit brings biotechnology to life and strongly supports curriculum learning outcomes. It is particularly effective for lessons covering:

• Genetic transformation and plasmids
• Gene expression and regulation
• Use of antibiotic selection
• Microbiology and aseptic technique
• Modern applications of genetic engineering in medicine and research

Highly visual, hands‑on, and curriculum‑aligned, the Transformation of E. coli with GFP Kit is an outstanding teaching resource that engages students, strengthens practical skills, and demonstrates how molecular biology is used in real scientific laboratories.