Edvotek A-maizing Editing: Using CRISPR to Improve Crops [80200]

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Explore cutting-edge biotechnology with this hands-on CRISPR-Cas9 simulation. Students will assume the role of plant geneticists working to develop corn crops capable of surviving and thriving, in a changing environment. This experiment will allow students to develop an understanding of CRISPR-Cas9 applications in the laboratory, cleave DNA, and examine their results after gel electrophoresis. Expt. 210
• Hands-On Crispr Cas 9 Simulation
• Explore this cutting edge technology with minimal equipment and class time requirements
• Create four guide RNAs (gRNAs), then use BLAST to verify and evaluate their specificity
• Test all four gRNAs plus control and analyse results using gel electrophoresis