Edvotek PCR Amplification of DNA – Classroom PCR Experiment Kit

The Edvotek PCR Amplification of DNA Kit introduces students to one of the most powerful techniques in modern molecular biology: Polymerase Chain Reaction (PCR). In this straightforward experiment, learners will generate billions of copies of a specific DNA sequence in as little as 90 minutes. By simply mixing template DNA and primers with ready‑to‑use PCR EdvoBeads™, students perform a complete PCR reaction without the need for complex reagent preparation.

Throughout the activity, students take samples after selected PCR cycles and analyse them using agarose gel electrophoresis, allowing them to visually track the increasing abundance of amplified DNA. Designed for 10 lab groups (2–4 students per group), this kit provides an accessible and engaging introduction to DNA technology and biotechnology skills.

The kit includes a comprehensive set of materials: PCR EdvoBeads™, LyphoPrimer™ Mix, DNA marker, LyphoTemplate™, TE buffer, UltraSpec‑Agarose™, FlashBlue™ Stain, SYBR® Safe DNA Stain, electrophoresis buffer, microcentrifuge tubes, and PCR tubes, along with full instructions. Required equipment includes adjustable micropipettes (5–50 μl), a thermal cycler, electrophoresis system, power supply, microwave or hot plate, UV transilluminator, and optionally a white light visualiser when using FlashBlue™ stain.

Key Features

• Easy‑to‑run PCR practical producing billions of DNA copies in 90 minutes
• Uses PCR EdvoBeads™ containing all essential PCR reagents
• Students analyse DNA amplification using gel electrophoresis
• Perfect for 10 student groups
• Includes primers, template DNA, DNA ladder, stains, buffers, agarose, tubes, and more
• Supports multiple stain options: FlashBlue™ (visible light) or SYBR® Safe (UV light)
• Clear results reinforce core concepts in molecular biology and biotechnology

You need: 5–50 μl micropipettes, tips, thermal cycler, electrophoresis tank, power supply, microwave or hot plate, UV transilluminator, white light visualiser (optional for FlashBlue™).

Ideal for School Science Lessons & Biotechnology Learning

Well suited for GCSE, KS5, and A‑Level Biology, this kit demonstrates one of the foundational methods used in genetics, forensics, medical diagnostics, and research laboratories worldwide. Students gain hands‑on experience with:

• The full PCR workflow from reaction setup to thermal cycling
• DNA amplification and the exponential increase of DNA fragments
• Electrophoresis and the visualisation of PCR products
• Good laboratory practice and micropipetting skills
• Understanding how PCR supports applications such as genetic testing, pathogen detection, and DNA profiling

Clear, hands‑on, and curriculum‑aligned, the Edvotek PCR Amplification of DNA Kit provides a powerful learning experience that helps students understand and perform one of the most essential techniques in modern molecular biology.