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Transformation Of E. Coli With Green Fluorescent Protein [1830]

Transformation Of E. Coli With Green Fluorescent Protein [1830]

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£63.48 Ex VAT

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Overview

Transformed cells take up a plasmid containing the GFP gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to long wave UV light. For 10 Lab Groups. Kit includes: instructions, BactoBeads™, E. coli GFP Host, supercoiled pFluoroGreen™, ampicillin, IPTG, CaCl2, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipettes, wrapped 10 ml pipette (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes


You Need: automatic micropipette (5-50 µl) and tips, two waterbaths (37°C and 42°C), thermometer, incubation oven (37°C), pipette pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light.


Typical Delivery Time: 1 - 3 Days

Details

Transformed cells take up a plasmid containing the GFP gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to long wave UV light. For 10 Lab Groups. Kit includes: instructions, BactoBeads™, E. coli GFP Host, supercoiled pFluoroGreen™, ampicillin, IPTG, CaCl2, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipettes, wrapped 10 ml pipette (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes You Need: automatic micropipette (5-50 ?l) and tips, two water baths (37ᄎC and 42ᄎC), thermometer, incubation oven (37ᄎC), pipette pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light.